DL-Dithiothreitol/DTT

ID:CH-2026-04-24
CAS No.: 3483-12-3

Specification

Product Name: DL-Dithiothreitol (DTT)
CAS No.: 3483-12-3
Molecular Formula: C₄H₁₀O₂S₂
Molecular Weight: 154.25 g/mol
Appearance: White to off-white crystalline powder
Purity: ≥ 99%

Description: A white free flowing powder, hygroscopic

Solubility: Free Soluble in water, Methanol and Methylene Chloride

Clarity:

a.) In Water: 5%(w/v) Solution in water is clear and colorless

b.) In Sodium Acetate:1 molar DTT solution in 0.01M sodium acetate at pH 5.2 should be clear and colorless

Identification by

a) IR Spectrum: IR Spectrum of sample exhibits maxima at the same wavelengths as that of a similar preparation of reference compound.

b) TLC: Rf value of the sample is comparable with that of the standard.

Melting Range:42-44 °C.

Related Substance

UV Spectroscopy: Content of Oxidized DTT (Dihydroxydithiane) NMT 0.4%

UV Absorbance

a) At 500nm: Absorbance of 0.1M Solution at 500nm NMT 0.035

b) At 280nm: Absorbance of 0.1M Solution at 280nm NMT 0.100

pH / Acidity: pH of 0.1M solution in water is between 4.0 and 6.0

Assay ( By lodimetry ): NLT 99%

Loss on Drying: < 0.5%

Description

DL-Dithiothreitol (DTT) is a widely used reducing agent in biochemical and molecular biology applications. It is particularly effective in breaking disulfide bonds within and between proteins, thereby maintaining proteins in their reduced (active) form.

DTT is commonly used in protein chemistry, enzymatic studies, and nucleic acid research, where it helps prevent oxidation and preserves the structural integrity of biomolecules. Its strong reducing capability makes it an essential reagent in laboratory protocols involving protein denaturation, SDS-PAGE sample preparation, and enzyme stabilization.

DL-Dithiothreitol/DTT