Designed to meet the demands of modern, large-scale manufacturers Capto Q’s shorter processing times can reduce exposure of the target protein.
Capto Q is designed for capture and intermediate purification of proteins from large feed volumes. It is composed of a rigid, high-flow agarose matrix modified with dextran surface extenders and a strong quaternary ammonium (Q) anion exchanger. The combination of high-volume throughput and high dynamic binding capacity for Capto Q raises the productivity in industrial downstream processes.
Capto Q withstands effective and rigorous cleaning-in-place (CIP) procedure and the chemical stability supports long resin lifetime.
| Chromatography technique | Ion Exchange Chromatography |
| BioProcess resin | Yes |
| Certificate of Analysis | Yes |
| Chemical stability | Commonly used aqueous buffers, 1 M acetic acid, 1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol and 70% ethanol |
| Ion Exchanger Type | Strong anion exchanger |
| Ionic Capacity | 0.16-0.22 mmol Cl-/ml medium |
| Ligand | Quaternary amine |
| Matrix | Highly cross-linked agarose with dextran surface extender |
| Particle size, d50V | ~90 μm |
| Pressure/Flow Specification | 0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height |
| Storage | 4 to 30°C, 20% Ethanol |
| pH stability, CIP | 2–14 |
| pH stability, operational | 2–12 |